GrainGenes Reference Report: JCS-19-101
Reference
JCS-19-101
Title
Identification of cereals using the polymerase chain reaction
Journal
Journal of Cell Science
Year
1994
Volume
19
Pages
101-106
Author
Ko H
[ Show all 6 ]
Abstract
Methods based upon the polymerase chain reaction (PCR) have been developed for distinguishing cereal species and cultivars DNA isolated from the leaves of germinating seedlings or grain gave similar results Primers designed to amplify the spacers between 5S ribosomal RNA genes were able to identify samples of maize, barley, sorghum, rye, rice, oat and wheat without any significant variation within these species Arbitrary 10-mers allowed distinction between cultivars of the same species, although some variation was found within cultivars, and some primers gave products characteristic of the cereal species These methods have potential for application in the analysis of the species and cultivar composition of cereal grain lots and cereal products
Keyword
cereals cultivar identification polymerase chain reaction species
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: JCS-19-101
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||