GrainGenes Reference Report: JCS-17-191
Reference
JCS-17-191
Title
Reversed-phase high-performance liquid chromatography of secalins: application to rye cultivar identification
Journal
Journal of Cell Science
Year
1993
Volume
17
Pages
191-201
Author
Kubiczek R Huebner F Bietz J
Abstract
Ethanol-extractable proteins (secalins) from whole-grain meal of: (a) the Polish rye cultivar Dankowskie Zlote grown in 1978-1984 with tent-type isolation during flowering; (b) the same cultivar grown in 1989 in a space-isolated plot; and (c) additional cultivars grown with tent isolation, were characterized and compared Methods of secalin extraction and analysis by reversed-phase high-performance liquid chromatography (RP-HPLC) were optimized These methods showed that, within the cultivar Dankowskie Zlote, secalin composition was nearly constant from year to year, and was largely independent of environment In later years, however, one additional secalin was present, possibly indicating more than one genetic pool within the cultivar RP-HPLC yielded distinct patterns for different cultivars, permitting their identification. These results suggest that RP-HPLC can be a valuable technique for rye breeding and genetical studies.
Keyword
cultivar
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GrainGenes Reference Report: JCS-17-191
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||