GrainGenes Reference Report: APM-79-941
Reference
APM-79-941
Title
Cloning and characterization of a novel exo-alpha-1,5-L: -arabinanase gene and the enzyme.
Journal
Applied Microbiology and Biotechnology
Year
2008
Volume
79
Pages
941-949
Author
Wong DW Chan VJ Batt SB
Abstract
A novel exo-alpha-1,5-L: -arabinanase gene (arn3) was isolated, cloned, and expressed in E. coli. The recombinant enzyme (ARN3) had a pH optimum of 6.0-7.0 and a pH 3.0-7.0 stability range. The temperature optimum was 50 degrees C with a stability less than or equal to 45 degrees C. The recombinant ARN3 cleaved carboxymethyl (CM)-arabinan, debranched arabinan, and linear arabinan at a decreasing rate and is inactive on sugar beet arabinan, wheat arabinoxylan, and p-nitrophenyl-alpha-L: -arabinofuranoside. The enzyme hydrolyzed debranched arabinan and synthetic arabino-oligosaccharides entirely to arabinose. The apparent K (m) and V (max) values were determined to be 6.2 +/- 0.3 mg/ml and 0.86 +/- 0.01 mg ml(-1) min(-1), respectively (pH 7.0, 37 degrees C, CM-arabinan). Multiple sequence alignment and homology modeling revealed unique short sequences of amino acids extending the loop involved in partial blocking of one end of the substrate-binding site on the surface of the molecule.
External Databases
http://dx.doi.org/10.1007/s00253-008-1504-4
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: APM-79-941
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
| ||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||