GrainGenes Reference Report: APM-40-306
Reference
APM-40-306
Title
Expression and secretion of wheat alpha-amylase in Kluyveromyces lactis
Journal
Applied Microbiology and Biotechnology
Year
1993
Volume
40
Pages
306-308
Author
Russell C Jarvis A Yu P Mawson J
Abstract
The plasmid pCR1 has been constructed to express a wheat alpha-amylase enzyme in Kluyveromyces lactis strains The construct is based on the vector pCXJ-kan1, which has been derived from pKD1, a native plasmid of K lactis var drosophilarum containing the essential regions for plasmid replication and stability Construct pCR1 produces an alpha-amylase by DNA isolated from a wheat cDNA clone and is controlled by a Saccharomyces cerevisiae PGK promoter
Keyword
alpha-amylase kluyveromyces-marxianus-var lactis wheat

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