GrainGenes Reference Report: JAP-46-357
Reference
JAP-46-357
Title
PstI)AFLP based markers for leaf rust resistance genes in common wheat
Journal
Journal of Applied Genetics
Year
2005
Volume
46
Pages
357-364
Author
Blaszczyk L Tyrka M Chelkowski J
Abstract
The aim of the present study was to detect candidate DNA markers for selected leaf rust resistance genes. A total number of 286 loci in the 'Thatcher' near-isogenic lines carrying resistance gene Lr1, Lr9, Lr10, Lr13, Lr19, Lr21, Lr24, Lr26, Lr28, Lr35, and Lr37 were screened for DNA polymorphism by the (PstI)AFLP method. A survey with 33 selective primers yielded 16 candidate markers. Further validation studies on cultivars characterized for the presence and absence of selected resistance genes confirmed specificity of markers for Lr24, Lr26 and Lr37. The AFLP-based marker P42-530 was successfully converted into an STS marker. The new marker was linked with the Lr37 -specific marker (CslVrga13) at the distance of 1.7 cM. The (PstI)AFLP method was found to be effective in the identification of DNA changes induced in hexaploid wheat by translocations from Agropyron elongatum, Secale cereale and Aegilops ventricosa.
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: JAP-46-357
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||