GrainGenes Reference Report: ISP-22-179
Reference
ISP-22-179
Title
Using real-time PCR to determine transgene copy number in wheat
Journal
Plant Molecular Biology Reporter
Year
2004
Volume
22
Pages
179-188
Author
Li ZW Hansen JL Liu Y Zemetra RS Berger PH
Abstract
Transgene copy number is usually determined by means of Southern blot analysis, which can be time consuming and labourious. In this study, quantitative real-time PCR was developed to determine transgene copy number in transgenic wheat. A conserved wheat housekeeping gene, puroindoline-b , was used as an internal control to calculate transgene copy number. Estimated copy number in transgenic lines using real-time quantitative PCR was correlated with actual copy number based on Southern blot analysis. Real-time PCR can analyse hundreds of samples in a day, making it an efficient method for estimating copy number in transgenic wheat
Keyword
control polymerase chain reaction transgenic plant
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: ISP-22-179
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||