GrainGenes Reference Report: HER-84-46
Reference
HER-84-46
Title
Chromosomal localization of cotransformed transgenes in the hexaploid cultivated oat Avena sativa L. using fluorescence in situ hybridization
Journal
Heredity
Year
2000
Volume
84
Pages
46-53
Author
Leggett JM Perret SJ Harper J Morris P
Abstract
Summary: Fluorescence in situ hybridization (FISH) was used to localize two transgenes (gus and bar), carried on plasmids pACT-1F and pUBA, respectively, on mitotic metaphase squashes of T1 plants of the cultivated hexaploid oat Avena sativa L. cotransformed by microprojectile bombardment of embryogenic callus. Among the eight progeny analysed by FISH in each of two lines, we detected plants null, hetero- and homozygous for the two genes in one line, and plants null and heterozygous for the two genes in the other line. Our results demonstrated that in the two independent transformation events, the gus and bar genes had inserted in the same position relative to each other. In each transformation event, the insertions occurred on D satellite (SAT) chromosomes bearing a C genome translocation
External Databases
Pubmed: 10692010
Keyword
avena
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GrainGenes Reference Report: HER-84-46
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||