Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: HER-76-166

[Submit comment/correction]

Reference	HER-76-166
Title	Two-dimensional electrophoresis of proteins as a source of monogenic and codominant markers for population genetics and mapping the expressed genome
Journal	Heredity
Year	1996
Volume	76
Pages	166-177
Author	Vienne D [ Show all 10 ]
Abstract	The positional polymorphism of polypeptides revealed using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was analysed in segregating families of four plant species, a gymnosperm (maritime pine), and three angiosperms (maize, barley and pea) All of the 170 variations scored had monogenic and codominant inheritance, making 2-D PAGE a quite abundant and cheap source of good-quality genetic markers Genetic mapping showed that the loci involved are well distributed on the chromosomes In particular, the construction of a composite map of the maize genome including 253 markers revealed that the protein loci are interspersed with the RFLP loci, and provide in some instances markers for chromosomal regions previously lacking molecular markers. In the context of the genome mapping projects, such markers are physiologically relevant in that they reveal loci whose transcripts are translated in the organ analysed.
Keyword	2-d-page [ Show all 18 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.