GrainGenes Reference Report: HDT-141-46
Reference
HDT-141-46
Title
Molecular cloning and comparative analysis of a y-type inactive HMW glutenin subunit gene from cultivated emmer wheat (Triticum dicoccum L.)
Journal
Hereditas (Lund)
Year
2004
Volume
141
Pages
46-54
Author
Sun M
[ Show all 9 ]
Abstract
Cultivated emmer (T. dicoccum, 2n=4x=28, AABB) is closely related to bread wheat and possesses extensive allelic variations in high-molecular-weight glutenin subunit (HMW-GS) composition. These alleles may be an important genetic resource for wheat quality improvement. To isolate and clone HMW-GS genes from cultivated emmer, 2 pairs of allele-specific (AS) polymerase chain reaction (PCR) primers were designed to amplify the coding sequence of y-type HMW-GS genes and their upstream sequences, respectively. The results showed that single bands of strong amplification were obtained through AS-PCR of genomic DNA from emmer. After cloning and sequencing, the complete sequence of coding and 5'-flanking regions of a y-type subunit gene at Glu-A1 locus was obtained. Nucleotide and deduced amino acid sequences analysis showed that this gene possessed a similar structure as the previously reported Ay gene from common wheat, and is hence designated as Ay1d. The distinct feature of the Ay1d gene is that its coding region contains 4 stop codons and its upstream region has a 85-bp deletion in the same position of the Ay gene, which are probably responsible for the silencing of y-type subunit genes at Glu-A1 locus. Phylogenetic analysis of HMW glutenin subunit genes from different Triticum species and genomes were also carried out
Keyword
allele
[ Show all 14 ]
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GrainGenes Reference Report: HDT-141-46
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||