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GrainGenes Reference Report: GNM-39-93

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Reference
GNM-39-93
Title
Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence
Journal
Genome
Year
1996
Volume
39
Pages
93-104
Author
Pedersen C
Rasmussen S
Linde-Laursen I
Abstract
The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed
Keyword
chromosome-analysis
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