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GrainGenes Reference Report: GNM-38-991

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Reference	GNM-38-991
Title	Mapping of digested and undigested random amplified microsatellite polymorphisms in barley
Journal	Genome
Year	1995
Volume	38
Pages	991-998
Author	Becker J Heum M
Abstract	The broad use of microsatellites as a tool for constructing linkage maps in plants has been limited by the need for sequence data to detect the underlying simple sequence repeats Therefore, random amplified microsatellite polymorphisms (RAMPs) were studied as an alternative approach for barley mapping Labelled (GA)n simple sequence repeat primers were combined with RAPD primers of different length and sequence to generate RAMPs To get additional polymorphisms (called dRAMPs), the obtained products were also analysed after digestion with MseI There were 0-11 polymorphisms found per primer combination Sixty RAMPs/dRAMPs identifying 40 new loci were mapped onto a barley RFLP map The new DNA markers are found on all chromosomes and they increased the length of the barley map by 174 cM to a total of 1270 cM. Interestingly, the RAMPs/dRAMPs caused stretching effects in genome areas where stretching was also observed for AFLPs.
Keyword	chromosome-maps [ Show all 9 ]

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