GrainGenes Reference Report: GNM-38-543
Reference
GNM-38-543
Title
Identification of RAPD markers linked to a gene governing cadmium uptake in durum wheat
Journal
Genome
Year
1995
Volume
38
Pages
543-547
Author
Penner GA Clarke J Bezte LJ Leisle D
Abstract
Temperature sweep gel electrophoresis in combination with random amplified polymorphic DNA analysis was employed to detect two markers for a single gene governing low cadmium uptake in western Canadian durum wheat (Triticum turgidum L. var. durum). Analysis of progeny derived from a cross of the high cadmium accumulating cultivar Kyle by the low cadmium accumulating cultivar Nile resulted in linkage estimates of 4.6 (OPC-20) and 21.2 (UBC-180) cM. The closest marker (OPC-20) was shown to be useful for making low cadmium uptake selections from two other sources of low cadmium, 'Biodur' and 'Hercules'. The marker was further used to determine the genetic basis of resistance in 20 introduced durum wheat lines. Within this diverse range of germplasm the marker was correlated with cadmium contents as expected in all but two cases. Plant breeding selection for low cadmium genotypes is hindered by the high cost of chemical determination of cadmium content. Marker assisted selection for a low cadmium uptake gene offers an effective alternative.Key words: cadmium, durum wheat, RAPD markers, marker assisted selection, temperature sweep gel electrophoresis.
External Databases
https://doi.org/10.1139/g95-070
Gene
cdu1 (Triticum) Cdu1 (Triticum)
Germplasm
Biodur
2 Point Data
cdu1/OPC-20
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: GNM-38-543
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||