GrainGenes Reference Report: GNM-36-1088
Reference
GNM-36-1088
Title
Rapid detection of 1B, 1BL/1RS heterozygotes in the development of homozygous 1BL/1RS translocation stocks of Triticum turgidum (2n = 4x =28)
Journal
Genome
Year
1993
Volume
36
Pages
1088-1091
Author
Williams M Muijeeb-Kazi A
Abstract
A biochemical marker was utilized to facilitate detection of chromosome 1B, 1BL/1RS translocation heterozygote plants in segregating backcross progenies during the development of 1BL/1RS homozygous lines in several Triticum turgidum L cultivars (2n = 4x = 28, AABB) Isoelectric focussing of glucose phosphate isomerase (GPI) on either pH 3.5-9.5 or 5.5-8.5 polyacrylamide gels facilitated the detection of 1B, 1BL/1RS translocation heterozygotes from the homozygous 1B or 1BL/1RS derivatives during each backcross of the heterozygote to the respective recurrent parent. The biochemical diagnostic procedure complements the more time consuming and cumbersome chromosome banding technique. This GPI diagnostic in durum 1BL/1RS development is also swifter than a similar stocks development in T. aestivum where both GPI and acid PAGE are essential.
Keyword
chromosome
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GrainGenes Reference Report: GNM-36-1088
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||