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GrainGenes Reference Report: GNM-36-731

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Reference
GNM-36-731
Title
Standard karyotype of Triticum longissimum and its cytogenetic relationship with T. aestivum
Journal
Genome
Year
1993
Volume
36
Pages
731-742
Author
Friebe B
Tuleen N
Jiang J
Gill BS
Abstract
C-banding polymorphism was analyzed in 17 accessions of Triticum longissimum from Israel and Jordan, and a generalized idiogram of this species was established. C-banding analysis was further used to identify two sets of disomic T. aestivum - T. longissimum chromosome addition lines and 13 ditelosomic addition lines and one monotelosomic (6S^l^L) addition line. C-banding was also used to identify T. aestivum - T. longissimum chromosome substitution and translocation lines. Two major nucleolus organizing regions (NORs) on 5S^l^ and ^S^l^ and one minor NOR on 1S^l^ were detected by in situ hybridization using a 18S-26S rDNA probe. Sporophytic and gametophytic compensation tests were used to determine the homoeologous relationships of T. longissimum chromosomes. The T. longissimum chromosomes compensate rather well and fertility was restored even in substitution lines involving wheat chromosomes 2A, 4B, and 6B that contain major fertility genes. Except for the deleterious gametocidal genes, T. longissimum can be considered as a suitable donor of useful genes for wheat improvement.
Keyword
C-banding
homoeology
in situ hybridization
Triticum aestivum
Triticum longissimum
Image
C-bands of Ae. longissima - 1
C-bands of Ae. longissima - 2
C-bands of CS - Ae. longissima

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