GrainGenes Reference Report: GNK-26-1258
Reference
GNK-26-1258
Title
Cloning and partial characterization of barley D hordein sequence
Journal
Genetika
Year
1990
Volume
26
Pages
1258-1268
Author
Pokrovskaya OV Mil'shina NV Anan'ev EV
Abstract
A number of clones containing major endosperm-specifically transcribed gene copies were selected from a cDNA library developed on the basis of barley endosperm mRNA. Approx. 30% of the recombinant clones carried sequences homologous to mRNA of various cereal storage proteins. Some of them appeared to be related to cDNA clones of wheat and barley storage proteins. The typical insert length range from 0.3 to 1.7 kB. A couple of clones among them were selected which revealed positive hydbridization with all probes used. The positive signals disappeared after stringent washing of the filters. The nucleotide sequences of two representatives of the group were determined and corresponding amino acid sequence deduced after subsequent computer analysis. The comparison with known cereal storage protein genes revealed relatively high homology level with the central part of wheat high molecular weight (HMW) glutenine subunit genes. The fact suggests the cloned gene to belong the barley D-hordein family.
Probe
pCHS2
Keyword
Amino acid sequence
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GrainGenes Reference Report: GNK-26-1258
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||