GrainGenes Reference Report: GCE-46-521
Reference
GCE-46-521
Title
Protein heterogeneity in European wheat landraces and obsolete cultivars
Journal
Genetic Resources and Crop Evolution
Year
1999
Volume
46
Pages
521-528
Author
Gregova E Hermuth J Kraic J Dotlacil L
Abstract
Summary: Identity and present degree of genetic homogeneity and heterogeneity, respectively of 52 European wheat accessions, maintained in the collection of wheat genetic resources, have been characterized using analyses of glutenins by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). Six of the analyzed wheat accessions were observed to be homogeneous, while 46 (88.5%) of them were heterogeneous in protein profiles. Heterogeneous accessions possessed 2 to 13 different protein lanes. Together, 17 high molecular weight glutenin subunit (HMW-GS) alleles have been found. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1, and Glu-D1 complex loci were 1, 7+9, and 2+12, respectively. However, also low frequented HMW-GS alleles or allelic combinations, such as 7+15, 13+16, 20, 6, 7, and 9 were observed. Furthermore, another new allele encoding HMW glutenin subunit with relative molecular weight 98.6 kDa has been found in one of the lines of the cultivar Eritrospermum 917. The Glu-score in the examined accessions varied in broad range, some of the lines reached the maximum value 10
Keyword
accessions
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GrainGenes Reference Report: GCE-46-521
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||