GrainGenes Reference Report: FBS-583-2865
Reference
FBS-583-2865
Title
Structural basis of the antifungal activity of wheat PR4 proteins.
Journal
FEBS Letters
Year
2009
Volume
583
Pages
2865-2871
Author
Bertini L Caporale C Testa M Proietti S Caruso C
Abstract
PR4 proteins possess antifungal activity against several pathogenic fungi suggesting a pivotal role in defence reactions against plant pathogen attack. We already showed that wheatwin1, a wheat PR protein of class 4, is endowed with ribonuclease activity. In this study we produced three mutants altering the active site and performed comparative analysis with the native protein also in the presence of the ribonuclease inhibitor 5'-ADP. We characterized the RNA binding site and its interaction with 5'-ADP by 3D modelling and docking studies. Moreover, in vitro antifungal assays have been carried out in order to study the relationship between antifungal and ribonuclease activities. Finally, localization of wheatwin1 in Fusarium culmorum spores was evaluated using fluorescence light microscope.
External Databases
http://dx.doi.org/10.1016/j.febslet.2009.07.045
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: FBS-583-2865
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||