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GrainGenes Reference Report: CRS-33-1201

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Reference	CRS-33-1201
Title	Linkage between endopeptidase Ep-D1d and a gene conferring leaf rust resistance (Lr19) in wheat
Journal	Crop Science
Year	1993
Volume	33
Pages	1201-1203
Author	McMillin D Johnson J Roberts J
Abstract	Biochemical markers linked to pest resistance genes would be useful to plant breeders This study was conducted to determine if biochemical markers could be found closely linked to genes conferring leaf rust resistance in wheat (Triticum aestivum L. Near-isogenic lines have been developed in 'Thatcher' for a number of genes, Lr1 to Lr31, conferring resistance to leaf rust, caused by Puccinia recondita Rob ex Desm Fifteen of these Thatcher near-isogenic lines were evaluated for the isozyme endopeptidase Two of the near-isogenic lines (Lr1 and Lr19) had endopeptidase phenotypes that differed from the Thatcher backcross parent. The endopeptidase phenotype observed in the near-isogenic line possessing Lr19 was completely different from any other phenotype normally observed in wheat. The leaf rust resistant Thatcher near-isogenic line possessing Lr19 was crossed with the leaf rust susceptible cultivar 'Ga 100' (PIS38257). The 59 F2 progeny were grown in the greenhouse and evaluated for leaf rust resistance and endopeptidase phenotype. All F2 progeny exhibiting the Lr19 endopeptidase phenotype were resistant to leaf rust. Maximum likelihood estimation, using the F2 phenotypic data, indicated a very tight linkage with a recombination value of 0.01 +/- 0.009 map units for the Lr19 and Ep-D1d loci. In addition, no recombinant F2 genotypes were found among the 30 F2 plants for which F3 progeny were obtained. Thus, the gene Lr19, which confers resistance to leaf rust, and the gene Ep-D1d which encodes. endopeptidase phenotype Ep-D1d, are linked. This linkage will enable rapid detection of seedlings possessing Lr19 in wheat improvement programs.
Keyword	disease resistance [ Show all 10 ]

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