GrainGenes Reference Report: CRS-33-525
Reference
CRS-33-525
Title
Fructan and sugars in 273 oat accessions
Journal
Crop Science
Year
1993
Volume
33
Pages
525-529
Author
Livingston D, III Elwinger G Weaver J
Abstract
Oat (Avena sativa L. accumulates less fructan during hardening than other winter cereals Little is known about the variability for fructan concentration after hardening in the genus Avena, however To investigate genetic variability, fructan was measured in 273 oat accessions grown under controlled conditions Dicktoo barley (Hordeum vulgare L. was included as a high degree of polymerization (DP) fructan checks. After 3 wk of hardening, plant stems (1 cm above the crown) were ground and extracted with water. Carbohydrates were separated by high performance liquid chromatography (HPLC) and quantified by comparison of peak areas in know standards to that in unknowns. On a fresh weight basis more than half the accession had total fructan (DP) > 2) levels not significantly (P < 0.05) different from Dicktoo barley, however, all but one accession had less (P < 0.05) high DP fructan (DP > 4) than Dicktoo. Based on the percentages of five carbohydrates, the accessions were grouped into eight clusters. One of the five species analyzed was alone in a cluster; the remaining species were spread across several clusters. Genetic variability for fructan concentration is apparently high enough in the accessions analyzed in this study to permit more detailed physiological studies with fewer accessions with a wide range of fructan content.
Keyword
avena sativa
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GrainGenes Reference Report: CRS-33-525
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||