GrainGenes Reference Report: CRL-54-1
Reference
CRL-54-1
Title
Characterization of complementary DNA clone for barley leaf glutamine synthetase
Journal
Carlsberg Research Communications
Year
1989
Volume
54
Pages
1-10
Author
Baima S Haegi A Stroman P Casadoro G
Type
Article
Language
English
Abstract
A barley cDNA clone (1182 bp) encoding chloroplastic glutamine synthetase was isolated with a heterologous cDNA probe of the gene specifying the enzyme from alfalfa. The clone, named pGS8, was found in a .lambda.gt11 cDNA library prepared from dark grown barley leaves even though the chloroplastic glutamine synthetase is absent from such leaves. In agreement therewith the clone hybridized in Northern blot analyses with a 1.7 kb mRNA species present in the poly A+ mRNA fraction of both dark grown and greened primary leaves of barley. The nucleotide sequence of the barley clone reveals 75% identity to the Phaseolus vulgaris and Pisum sativum clones encoding chloroplastic glutamine sythetase, while only 69% identity is observed in comparisons with the clones specifying the cytosolic isozymes. At the amino acid level 85% identity is found between the deduced barley glutamine synthetase sequence and that of the corresponding chloroplastic isoenzymes from bean and pea. The chloroplastic glutamine synthetases contain conserved cysteins in the putative ATP and substrate binding sites. In the cytosolic forms these positions are occupied by alanine residues.
Probe
pGS8
Keyword
Amino acid sequence Molecular sequence data Nucleotide sequence Phaseolus vulgaris Pisum sativum
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CRL-54-1
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||