GrainGenes Reference Report: CRL-51-445
Reference
CRL-51-445
Title
Construction of a yeast vector directing the synthesis and release of barley 1-3 1-4-beta glucanase
Journal
Carlsberg Research Communications
Year
1986
Volume
51
Pages
445-458
Author
Jackson EA Ballance GM Thomsen KK
Type
Article
Language
English
Abstract
Two cloned cDNA sequences from barley aleurone, representing complementary parts of a barley (1 .fwdarw. 3.1 .fwdarw. 4)-.beta.- glucanase gene were joined together and fused in frame to a DNA fragment encoding the mouse .alpha.-amylase signal peptide. The triplet determining the amino terminal amino acid residue of native .beta.-glucanase was changed from ATC (isoleucine) to CTA (leucine) in order to accommodate the .alpha.-amylase signal sequence in the correct reading frame. This chimaeric cDNA was inserted in the yeast expression vector pMA 56 behind the promoter from the yeast alcohol dehydrogenase I gene. Yeast cells harbouring such plasmids synthesize .beta.-glucanase and secrete the enzyme to the culture medium.
Probe
pBA170 pYBAG17
Keyword
Cloned dna sequence brewing
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CRL-51-445
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||