GrainGenes Reference Report: CRL-51-203
Reference
CRL-51-203
Title
Identification and characterization of a complementary DNA clone for cytosolic glyceraldehyde-3-phosphate dehydrogenase in barley
Journal
Carlsberg Research Communications
Year
1986
Volume
51
Pages
203-210
Author
Chojecki J
Type
Article
Language
English
Abstract
The nucleotide sequence of a cDNA clone for barley glyceraldehyde-3- phosphate dehydrogenase (GAPDH) has been determined. The cDNA is 1147 base pairs long, encoding 305 amino-acids of the GAPDH polypeptide. Comparison of the amino acid sequence, predicted from the DNA sequence, with GAPDH sequences available for other organisms reveals a high degree of sequence conservation, particularly in regions of supposed importance for tertiary structure and activity. Hybridisation of the cDNA insert to barley genomic DNA indicates that there is a single copy of the gene per haploid genome. The expression of the GAPDH gene in the scutellum during germination, as detected by RNA hydridisation analysis, probably reflects the involvement of the enzyme at key steps in both the glycolytic and gluconeogenic pathways.
Probe
pc gld-1 pc1-240
Keyword
Nucleotide sequence
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CRL-51-203
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||