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GrainGenes Reference Report: CRC-33-673

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Reference	CRC-33-673
Title	Demonstration of Aegilops biuncialis chromosomes in a wheat background by genomic in situ hybridization (GISH) and identification of U chromosomes by FISH using GAA sequences
Journal	Cereal Research Communications
Year	2005
Volume	33
Pages	673-680
Author	Molnar I Schneider A Molnar-Lang M
Abstract	The aims of the study were the optimisation of genomic in situ hybridization (GISH) and the use of a GAA satellite sequences as a fluorescence in situ hybridization (FISH) probe to discriminate Aegilops biuncialis and wheat chromosomes. The application of genomic DNA from the diploid progenitors, Ae. umbellulata and Ae. comosa, as a probe with a blocking ratio of 1:200 resulted in the clear visualization of Ae. biuncialis chromosomes. Based on the strong hybridization signals of the PCR-amplified GAA satellite sequences it was possible to identify chromosomes 1U, 2U, 4U and 5U of Ae. umbellulata. The improved GISH and GAA idiogram presented here allow the Ae. biuncialis chromatin to be traced in more detail during chromosome-mediated gene transfer to wheat.

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