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GrainGenes Reference Report: CRC-32-355

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Reference	CRC-32-355
Title	Identification of germin isoforms in wheat callus
Journal	Cereal Research Communications
Year	2004
Volume	32
Pages	355-361
Author	Calskan M Turet-Sayn M Turan C Cuming AC
Abstract	Application of auxin causes cells of the wheat embryo to undergo dedifferentiation and form callus tissue. The synthesis of germin isoforms (G, Gprime and psiG) has been selectively associated with different phases of embryonic development and callus formation. G and Gprime germin isoforms are found to be associated with germination. psiG is an embryogenesis related germin isoform. It is clearly important to distinguish whether callus induction caused a precocious initiation of embryonic maturation-specific pseudogermin synthesis, or whether it effected a switch in the pattern of protein synthesis, eliciting accumulation of the normally germination-related germin proteins. To distinguish between these alternatives, it is necessary to identify the germin protein isoforms accumulated during callus formation. Following the electrophoretic separation of callus protein extracts, the position of germin isomers were detected by Western-blotting. The immunoreactive polypeptides accumulating in callus tissue were determined to be exclusively germin, rather than psiG
Keyword	auxin [ Show all 12 ]

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