Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: CCM-81-561

[Submit comment/correction]

Reference	CCM-81-561
Title	Rapid identification of HMW glutenin subunits from different hexaploid wheat species by acidic capillary electrophoresis
Journal	Cereal Chemistry
Volume	81
Pages	561-566
Author	Yan YM [ Show all 10 ]
Abstract	High molecular weight glutenin subunits (HMW-GS) from three hexaploid wheat species (AABBDD, 2n=6x=42, Triticum aestivum L., T. spelta L., and T. compactum L.) were separated and identified by acidic capillary electrophoresis (A-CE) with phosphate-glycine buffer (pH 2.5) in uncoated fused-silica capillaries (50 microm, i.d. x 25.5 cm) at 12.5 kV and 40(deg)C. The rapid separations (<15 min) of HMW-GS with good repeatability (RSD<2%) were obtained using a fast capillary rising protocol. All 17 HMW-GS analysed could be well separated and their relative migration orders were ranked. In particular, the good quality subunit pair 5+10 could be differentiated from poor quality subunit pair 2+12. In addition, the other three allelic pairs of 13+16, 17+18, and 7+8 subunits that were considered to have positive effects on dough properties, as well as three pairs of novel subunits 13+22*, 13*+19*, and 6.1+22.1 detected from spelt and club wheat, can also be readily separated and identified. An additional protein subunit presented in Chinese bread wheat cultivar Jing 411 and club wheat TRI 4445/75, respectively, was detected by both A-CE and 2-D gel electrophoresis (A-PAGE x SDS-PAGE), for which further identification is needed
Keyword	electrophoresis wheat

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.