GrainGenes Reference Report: CCM-77-11
Reference
CCM-77-11
Title
Distribution of peroxidases in durum wheat (Triticum durum)
Journal
Cereal Chemistry
Year
2000
Volume
77
Pages
11-17
Author
Fraignier M Michaux-Ferriere N Kobrehel K
Abstract
A biochemical study of the main durum wheat milling fractions (bran, embryo, and semolina) showed that peroxidases (POD) were present in multiple forms in the kernel and appeared to be tissue specific: one form for the embryo, one for the endosperm, one for the subaleuronic layer, and one for the outer layers Large varietal differences were found regarding both the composition and the POD activity POD activity, detected by diaminobenzidine, was found mainly in the cell wall of the subaleurone layer and inside some specific, differentiated cells of the embryo Immunolocalization with antibodies of durum wheat POD showed the presence of POD in several layers of the pericarp (epidermis) and the seed coat (testa), in the embryo, and also in the endosperm In this latter tissue, the staining intensity decreased gradually from the outer layers toward the center of the kernel. The localization of POD in durum wheat kernel suggests specific functions for different forms.
Keyword
chemical composition
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GrainGenes Reference Report: CCM-77-11
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||