GrainGenes Reference Report: MBR-38:14
Reference
MBR-38:14
Title
Detection of QTLs controlling alpha-amylase activity in a diversity panel of 343 barley accessions.
Year
2018
Volume
38
Author
Sato K Hisano H Matsumoto S Zhou T-S Kihara M
Abstract
The alpha-amylase activity of cultivated barley is critically important to the brewing industry. Here, we surveyed variation in malt alpha-amylase activity in 343 cultivated barley accessions from around the world. Population structure analysis based on genotype data at 1536 SNPs clustered these accessions into two groups, one comprising South-East Asian and Ethiopian accessions and one group containing the other accessions. A genome-wide association study identified significant quantitative trait loci (QTLs) for alpha-amylase activity on all seven chromosomes of barley. Accessions showing high and low alpha-amylase activity were crossed with the high-quality Japanese malting barley cv. Harun Nijo to develop F2 mapping populations. We identified two QTLs on chromosome 6H in a cross between Haruna Nijo (high activity) x Weal (highest activity). Single QTLs were identified each on 3H, 4H, and 5H from a cross between Haruna Nijo (high activity) x VLB-1 (low activity), indicating that the high alpha-amylase activity in Haruna Nijo might be derived from loci on these chromosomes. The addition of the high alpha-amylase activity QTL alleles from chromosome 6H in cv. Weal further increased the alpha-amylase activity conferred by alleles of Haruna Nijo. These results demonstrate that a target haplotype can be successfully improved using a strategy comprising diversity analysis of ex situ collections followed by introducing effective new alleles.
External Databases
https://doi.org/10.1007/s11032-017-0773-y
Cited in
Du B et al. (2024) Detection of consensus genomic regions and candidate genes for quality traits in barley using QTL meta-analysis Frontiers in Plant Science 14.
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: MBR-38:14
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||