Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: NGN-54-227

[Submit comment/correction]

Reference	NGN-54-227
Title	Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning
Journal	Nature Genetics
Year	2022
Volume	54
Pages	227-231
Author	Athiyannan N [ Show all 13 ]
Abstract	The cloning of agronomically important genes from large, complex crop genomes remains challenging. Here we generate a 14.7 gigabase chromosome-scale assembly of the South African bread wheat (Triticum aestivum) cultivar Kariega by combining high-fidelity long reads, optical mapping and chromosome conformation capture. The resulting assembly is an order of magnitude more contiguous than previous wheat assemblies. Kariega shows durable resistance to the devastating fungal stripe rust disease1. We identified the race-specific disease resistance gene Yr27, which encodes an intracellular immune receptor, to be a major contributor to this resistance. Yr27 is allelic to the leaf rust resistance gene Lr13; the Yr27 and Lr13 proteins show 97% sequence identity2,3. Our results demonstrate the feasibility of generating chromosome-scale wheat assemblies to clone genes, and exemplify that highly similar alleles of a single-copy gene can confer resistance to different pathogens, which might provide a basis for engineering Yr27 alleles with multiple recognition specificities in the future.
External Databases	https://www.nature.com/articles/s41588-022-01022-1
Gene	Yr27 (Triticum)
Germplasm	Kariega
Locus	Yr27
Probe	KASPYr27
Sequence	KASPYr27

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.