GrainGenes Reference Report: CAM-7-777
Reference
CAM-7-777
Title
The application of the AFLP method to determine the purity of homozygous lines of barley ( Hordeum vulgare L.)
Journal
Cellular and Molecular Biology Letters
Year
2002
Volume
7
Pages
777-783
Author
Oleszczuk S Zimny J Bednarek PT
Abstract
Amplified fragment length polymorphism of DNA has been used to analyse the equality of plants obtained from isolated microspores. Although the control parental material was regarded as being highly homozygous, the analysis of the banding patterns of single plants showed a certain level of polymorphism. The analysis of regenerants with a doubled chromosome number did not show any diversity within the progeny of a single line. The differences in banding patterns coming from single plants were only observed in microspore donor lines. These results have proven the high purity of homozygous lines obtained via androgenesis from isolated microspores
External Databases
Pubmed: 12378238
Keyword
acclimatization
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CAM-7-777
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||