GrainGenes Reference Report: CRC-33-439
Reference
CRC-33-439
Title
Validation of allele specific primers for identification of Rht genes among Indian bread wheat varieties.
Journal
Cereal Research Communications
Year
2005
Volume
33
Pages
439-446
Author
Nalini E Bhagwat SG Jawali N
Abstract
The dwarfing genes Rht-Blb and Rht-Dlb were incorporated into Indian bread wheats during the 'green revolution'. This led to a dramatic increase in yield as well as production of wheat. Although the presence of dwarfing genes is important, their status among the released wheat varieties is generally not known. We present an analysis of the two dwarfing genes using the PCR based 'perfect markers' in bread wheat (Triticum aestivum L.) varieties, for the first time in 45 Indian, and some non-Indian wheats. Most of the varieties analyzed had either Rht-Blb or Rht-Dlb or both. The results obtained for the varieties with known Rht status validated the perfect markers. Analysis of a F2 population segregating for the dwarfing genes also confirmed the utility of these markers in genetic analysis. Analysis among some of the varieties for the GA response phenotype is also presented. The results showed that the 'perfect markers' are robust enough to be used among Indian varieties.
External Databases
DOI: 10.1556/CRC.33.2005.2-3.104
Allele
Rht-D1d (Triticum)
Gene Class
Reduced height
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CRC-33-439
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||