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GrainGenes Reference Report: AAH-45-265

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Reference	AAH-45-265
Title	Mapping frost resistance loci in wheat and comparative mapping with other cereals.
Journal	Acta Agronomica Hungarica
Year	1997
Volume	45
Pages	265-270
Author	Snape JW [ Show all 6 ]
Abstract	Identifying and mapping the individual genes controlling frost resistance in wheat is difficult because effects are quantitative in nature and require precise and repeatable experimental conditions. Nevertheless, through the use of precise genetic stocks combined with RFLP markers, particularly recombinant substitution lines, progress has been made. In this way the Fr I gene on chromosome SA was mapped. This analysis has now been extended to chromosome SD, to map the locus, Frl, whose chromosomal location was previously indicated by chromosome assay. However, the very low levels of RFLP for this chromosome has made mapping difficult and progress has required the use of the new molecular marker system, AFLP to fill gaps between 'anchor' RFLPs. Analysis confirms that Fr2 is located on the long arm of chromosome 5D, is homocologous to Fr1, and linked, but separable, from Vrn3. A clear homocologous relationship to loci on chromosome 7(5H) of barley, and 5R of rye has been shown by cross-referencing maps containing common RFLP marker loci. This comparative genetic analysis is now being extended to other species, particularly rice, so that a strategy for positional cloning of the presumptive loci can be developed.
Gene	Fr1 (Triticum) Fr2 (Triticum)

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