GrainGenes Reference Report: GNM-51-426
Reference
GNM-51-426
Title
Mapping of a major locus controlling seed dormancy using backcrossed progenies in wheat (Triticum aestivum L.)
Journal
Genome
Year
2008
Volume
51
Pages
426-432
Author
Torada A Koike M Ikeguchi S Tsutsui I
Abstract
Seed dormancy is an important factor regulating preharvest sprouting (PHS) but is a complex trait for genetic analysis. We previously identified a major quantitative trait locus (QTL) controlling seed dormancy on the long arm of chromosome 4A (4AL) in common wheat. To transfer the QTL from the dormant lines 'OS21-5' and 'Leader' into the Japanese elite variety 'Haruyokoi', which has an insufficient level of seed dormancy, backcrossing was carried out through marker-assisted selection (MAS) using PCR-based codominant markers. Nineteen BC5F2 plants with homozygous alleles of 'OS21-5' or 'Haruyokoi' were developed and evaluated for seed dormancy under greenhouse conditions. The seeds harvested from plants with 'OS21-5' alleles showed a clearly high level of dormancy compared with seeds from plants with 'Haruyokoi' alleles. Additionally, the dormancy phenotype of BC3F3 seeds harvested from 128 BC3F2 plants with homozygous alleles of 'Leader' or 'Haruyokoi' showed a clear difference between these alleles. The QTL on 4AL confers a major gene, Phs1, which was mapped within a 2.6 cM region. The backcrossed lines developed in this study can be important sources for improving PHS resistance in Japanese wheat and for analyzing the mechanism of seed dormancy. MAS was useful for the development of near-isogenic lines in this complex trait, to facilitate the molecular dissection of genetic factors.
External Databases
https://doi.org/10.1139/G08-007
Gene
phs1 (Triticum) Phs1 (Triticum)
Gene Class
Pre-harvest sprouting
Germplasm
Haruyokoi*6/Leader Haruyokoi*6/Os21-5 Haruyokoi Leader Os21-5
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: GNM-51-426
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||