GrainGenes Reference Report: MBR-36:85
Reference
MBR-36:85
Title
Development and verification of wheat germplasm containing both Sr2 and Fhb1
Journal
Molecular Breeding
Year
2016
Volume
36
Author
Zhang X Rouse MN Nava IC Jin Y Anderson JA
Abstract
Fusarium head blight (FHB) and stem rust are two devastating fungal diseases of common wheat prevalent worldwide. Fhb1 is a well-known major quantitative trait locus effective against FHB, and Sr2 is the most widely characterized and deployed wheat stem rust adult plant resistance gene. These genes are linked in repulsion phase, approximately 3 cM apart, on chromosome 3B. To obtain wheat germplasm containing both Fhb1 and Sr2 genes, we developed and screened two F2 populations using tightly linked DNA markers, Xgwm533 and csSr2 for Sr2, and UMN10, and a newly designed marker UMN10v2 for Fhb1. Among 1949 F2 plants, 43 were identified with Fhb1 and Sr2 in coupling phase. After screened with vernalization gene markers, 59 F3 spring wheat lines with homozygous Fhb1 and Sr2 were obtained. All these lines showed significantly lower FHB severity than susceptible checks (P < 0.01) and similar FHB severity to the resistant checks (P > 0.61) in the field. And these lines were susceptible as seedlings in greenhouse assays, but resistant as adult plants to race QTHJC in the stem rust nursery. Field disease resistance evaluation verified that all these recombinant lines showed moderate or high levels of resistance to both FHB and stem rust. This germplasm would benefit efforts to breed for resistance to FHB and stem rust, and the procedure to obtain recombinants is adaptable to other wheat breeding programs.
External Databases
https://doi.org/10.1007/s11032-016-0502-y
Gene
Fhb1 (Triticum) Sr2 (Triticum)
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: MBR-36:85
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||