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GrainGenes Reference Report: GNB-20:284

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Reference	GNB-20:284
Title	TRITEX: chromosome-scale sequence assembly of Triticeae genomes with open-source tools
Journal	Genome Biology
Year	2019
Volume	20:284
Author	Monat C [ Show all 18 ]
Abstract	Chromosome-scale genome sequence assemblies underpin pan-genomic studies. Recent genome assembly efforts in the large-genome Triticeae crops wheat and barley have relied on the commercial closed-source assembly algorithm DeNovoMagic. We present TRITEX, an open-source computational workflow that combines paired-end, mate-pair, 10X Genomics linked-read with chromosome conformation capture sequencing data to construct sequence scaffolds with megabase-scale contiguity ordered into chromosomal pseudomolecules. We evaluate the performance of TRITEX on publicly available sequence data of tetraploid wild emmer and hexaploid bread wheat, and construct an improved annotated reference genome sequence assembly of the barley cultivar Morex as a community resource.
External Databases	https://doi.org/10.1186/s13059-019-1899-5
Locus	HORVU.MOREX.r2.1HG0000010.1 [ Show all 63658 ]
Map Data	Barley, 2019, IBSC Morex v2
Probe	HORVU.MOREX.r2.1HG0000010 [ Show all 62900 ]
Sequence	HORVU.MOREX.r2.1HG0000010.1 [ Show all 63658 ]

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