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GrainGenes Reference Report: XWR-18-257

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Reference	XWR-18-257
Title	BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance
Contained in	Wheat Rust Diseases.
Year	2018
Author	Huang L
Abstract	Virus-induced gene silencing (VIGS) is a widely used reverse genetics tool to knock down genes in plants transiently without transformation. The assay has been successfully used to downregulate the transcript abundance of a target gene at almost any plant developmental stages in any tissues. Here, we describe the VIGS assay using a barley stripe mosaic virus (BSMV) for functional genomics analysis in wheat with the focus on genes involved in rust resistance.
External Databases	https://doi.org/10.1007/978-1-4939-7249-4_22

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