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GrainGenes Reference Report: FIG-8-211

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Reference	FIG-8-211
Title	Gene expression in a starch synthase IIa mutant of barley: changes in the level of gene transcription and grain composition.
Journal	Functional and Integrative Genomics
Year	2008
Volume	8
Pages	211-221
Author	Clarke B [ Show all 6 ]
Abstract	The barley shrunken grain mutant M292 has a novel high-amylose starch phenotype caused by a mutation in the starch synthase IIa gene (SsIIa) located at the starch excess-6 (sex6) locus on chromosome 7H of barley. The loss of SSIIa enzyme activity leads to a decrease in amylopectin synthesis to less than 20% of the levels found in wild-type grains. Detailed composition analysis indicates that the contents of protein, non-starch polysaccharides, lipid, sucrose and hexoses, and fructo-oligosaccharides are increased in mature M292 grain compared to wild type. Using a microarray analysis, we characterize the differences between the transcription profiles of wild-type and mutant barley endosperms at mid-grain fill. The expression changes include genes involved in carbon storage, stress-related genes, and a number of transcripts with unassigned function. The changes in gene expression are discussed in terms of the altered grain composition of the mutant seed.
External Databases	http://dx.doi.org/10.1007/s10142-007-0070-7

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