GrainGenes Reference Report: FIG-11-49
Reference
FIG-11-49
Title
Wheat (T. aestivum) sucrose synthase 2 gene (TaSus2) active in endosperm development is associated with yield traits.
Journal
Functional and Integrative Genomics
Year
2010
Volume
11
Pages
49-61
Author
Jiang QY Hou J Hao CY Dong YS Zhang XY
Abstract
Sucrose synthase catalyzes the reaction sucrose_+_UDP___UDP-glucose_+_fructose, the first step in the conversion of sucrose to starch in endosperm. Previous studies identified two tissue-specific, yet functionally redundant, sucrose synthase (SUS) genes, Sus1 and Sus2. In the present study, the wheat Sus2 orthologous gene (TaSus2) series was isolated and mapped on chromosomes 2A, 2B, and 2D. Based on sequencing in 61 wheat accessions, three single-nucleotide polymorphisms (SNPs) were detected in TaSus2-2B. These formed two haplotypes (Hap-H and Hap-L), but no diversity was found in either TaSus2-2A or TaSus2-2D. Based on the sequences of the two haplotypes, we developed a co-dominant marker, TaSus2-2Btgw, which amplified 423 or 381-bp fragments in different wheat accessions. TaSus2-2Btgw was located between markers Xbarc102.2 and Xbarc91 on chromosome 2BS in a RIL population from Xiaoyan 54___Jing 411. Association analysis suggested that the two haplotypes were significantly associated with 1,000 grain weight (TGW) in 89 modern wheat varieties in the Chinese mini-core collection. Mean TGW difference between the two haplotypes over three cropping seasons was 4.26 g (varying from 3.71 to 4.94 g). Comparative genomics analysis detected major kernel weight QTLs not only in the chromosome region containing TaSus2-2Btgw, but also in the collinear regions of TaSus2 on rice chromosome 7 and maize chromosome 9. The preferred Hap-H haplotype for high TGW underwent very strong positive selection in Chinese wheat breeding, but not in Europe. The geographic distribution of Hap-H was perhaps determined by both latitude and the intensity of selection in wheat breeding.
External Databases
http://link.springer.com/article/10.1007%2Fs10142-010-0188-x
Keyword
haplotype sucrose synthase 2 thousand grain weight Triticum aestivum
Summarized in
MAS Wheat. Functional Markers for genes affecting agronomic traits in wheat MAS Wheat. Marker Assisted Selection in Wheat.
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: FIG-11-49
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||