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GrainGenes Reference Report: BPY-58-33

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Reference	BPY-58-33
Title	A high resolution H-1 magic angle spinning NMR study of high-M-r subunit of wheat glutenin
Journal	Biopolymers
Year	2001
Volume	58
Pages	33-45
Author	Alberti E [ Show all 7 ]
Abstract	Summary: This work describes the application of 1H magic angle spinning (MAS) nmr to the study of hydrated 1Dx5 wheat high-Mr subunit. 1Dx5 is a water-insoluble 88 kDa protein, associated with good baking performance, and whose structure in the solid and low-hydration states is not known. High-resolution MAS (HR-MAS) results in a threefold resolution improvement of the 1H spectra of the hydrated wheat protein, compared to standard MAS. The spectral resolution achieved enables, for the first time, two-dimensional nmr methods to be employed for the study of hydrated 1Dx5 and the assignment of the spectrum to be carried out on the basis of total correlated spectroscopy and 13C/1H correlation experiments. Considerable shifts are observed for some resonances, relative to the chemical shifts of amino acids in solution, indicating that specific interactions occur in the hydrated protein network. Two main environments are identified for glutamine residues, Q1 and Q2, and these were characterized in terms of possible conformation and relative dynamics, with the basis of comparison between the single 90 degrees spectrum and the Carr-Purcel-Heiboom-Gill (CPMG) spectrum. The Q1 residues are proposed to be situated in protein segments that adopt the beta-sheet conformation and that remain relatively hindered, possibly by hydrogen bonds involving the glutamine amide groups. On the other hand, Q2 residues are proposed to be situated in a more mobile environment, adopting a looser conformation, possibly a beta-turn conformation. Based on the proximity of the Q2 residues with glycine residues, as viewed by the nuclear Overhauser effect spectroscopy experiment, it is proposed that the protein segments that form the more mobile (or loop) sections of the network are rich in both glutamine and glycine residues
External Databases	Pubmed: 11072227
Keyword	amino acid [ Show all 35 ]

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