GrainGenes Reference Report: BPL-48-179
Reference
BPL-48-179
Title
Agrobacterium-mediated transformation and plant regeneration of Triticum aestivum L
Journal
Biologia Plantarum
Year
2004
Volume
48
Pages
179-184
Author
Mitic N Nikolic R Ninkovic S Miljus-Djukic J Neskovic M
Abstract
The use of two A. tumefaciens strains for transformation of T. aestivum cv. Vesna was studied. Immature embryos, isolated 15 days after pollination, were cocultivated with the super-binary LBA4404/pTOK233 and the binary AGL1/pDM805 vectors. While the transient GUS-intron expression was high (69.9 and 80.0%), the number of plants regenerated on selective media containing hygromycin or phosphinotricin did not exceed 0.4 and 0.13%, respectively. Nevertheless, the regenerated plants were fertile and produced seeds. The T SUB 0 plants, as well as the T SUB 1 seedlings, displayed the activity in the beta-glucuronidase histochemical assay and a positive signal in PCR analysis for the presence of uidA gene sequences in their genomes. The data suggest that the transformation of wheat cv. Vesna with both Agrobacterium strains is feasible
Keyword
agrobacterium
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GrainGenes Reference Report: BPL-48-179
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||