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GrainGenes Reference Report: EUP-104-181

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Reference	EUP-104-181
Title	Genetic diversity within spelta and macha wheats based on RAPD analysis.
Journal	Euphytica
Year	1998
Volume	104
Pages	181-189
Author	Cao WG Hucl P Scoles G Chibbar RN
Abstract	Crop germplasm collections contain a considerable percentage of misclassified accessions which may affect the use of germplasm for agricultural crop improvement. The objective of this study was to determine if random amplified polymorphic DNA (RAPD) analysis could be used to reclassify misclassified Triticum accessions. Twelve accessions suspected to be misclassified, based on morphological characters, as either macha or vavilovii wheat were studied using RAPD and cytological analyses. In the RAPD analysis, a dendrogram, based on Jaccard genetic similarity coefficients, grouped 5 dicoccum-like, 1 timopheevii-like, and 6 monococcum-like accessions with Triticum dicoccum, T. timopheevii, and T. monococcum accessions, respectively. These results were confirmed by the cytological analysis. A RAPD marker specific to the D genome was also detected. This study suggests that RAPD analysis can be used to classify germplasm and to distinguish some species in Triticum.
External Databases	Agricola: 2010-14060
Keyword	chiasma frequency [ Show all 19 ]

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