GrainGenes Reference Report: EMB-14-5753
Reference
EMB-14-5753
Title
Cholera toxin elevates pathogen resistance and induces pathogenesis-related gene expression in tobacco.
Journal
The EMBO Journal
Year
1995
Volume
14
Pages
5753-5761
Author
Beffa R
[ Show all 9 ]
Abstract
In animals, plants and fungi, cholera toxin (CTX) can activate signalling pathways dependent on heterotrimeric GTP binding proteins (G-proteins) We transformed tobacco plants with a chimeric gene encoding the A1 subunit of CTX regulated by a light-inducible wheat Cab-1 promoter Tissues of transgenic plants expressing CTX showed greatly reduced susceptibility to the bacterial pathogen Pseudomonas tabaci, accumulated high levels of salicylic acid (SA) and constitutively expressed pathogenesis-related (PR) protein genes encoding PR-1 and the class II isoforms of PR-2 and PR-3 In contrast, the class I isoforms of PR-2 and PR-3 known to be induced in tobacco by stress, by ethylene treatment and as part of the hypersensitive response to infection, were not induced and displayed normal regulation. In good agreement with these results, microinjection experiments demonstrated that CTX or GTP-gamma-S induced the expression of a PR1-GUS reporter gene but not that of a GLB-GUS reporter gene containing the promoter region of a gene encoding the class I isoform of PR-2. Microinjection and grafting experiments strongly suggest that CTX-sensitive G-proteins are important in inducing the expression of a subset of PR genes and that these G-proteins act locally rather than systemically upstream of SA induction.
Keyword
bacterial-toxins
[ Show all 16 ]
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GrainGenes Reference Report: EMB-14-5753
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||