GrainGenes Reference Report: CPP-26-89
Reference
CPP-26-89
Title
Development of sequence-characterized amplified region (SCAR) markers for resistance gene Pc94 to crown rust in oat
Journal
Canadian Journal of Plant Pathology
Year
2004
Volume
26
Pages
89-96
Author
Chong J Reimer E Somers D Aung T Penner GA
Abstract
Crown rust, caused by Puccinia coronata f. sp. avenae, is an important disease of oat (Avena sativa). Pyramiding effective resistance from diverse sources is one strategy to provide more durable control of crown rust. The objective of this study was to develop a DNA marker for the resistance gene Pc94 to crown rust derived from the diploid oat Avena strigosa. Amplified fragment length polymorphism generated two DNA fragments that were associated with the resistance gene Pc94 in a F2 population derived from a cross between the rust-susceptible oat 'Calibre' and a near-isogenic hexaploid oat line, S42, possessing Pc94. One of the fragments was successfully converted into two sequence-characterized amplified region (SCAR) markers linked to the Pc94 gene in the Calibre/S42 F2 population. Specificity of the SCAR markers to the Pc94 gene was confirmed in three other populations. The estimated distance between the SCAR markers and Pc94 ranged from 0.9 to 3.4 centi-Morgan. These SCAR markers developed for the Pc94 gene will be valuable tools for pyramiding this gene with other resistance genes to crown rust in marker-assisted breeding programs when crown-rust races with the appropriate virulence combinations are not available to detect the genes being introgressed.
External Databases
https://doi.org/10.1080/07060660409507118
Gene
Pc-94 (Avena)
Germplasm
S42 (oat)
Locus
Pc94 SCAR94-1 SCAR94-2
Map Data
Oat-2004-CalxS42 Oat-2004-MS68xS42
Probe
SCAR94-1 SCAR94-2
Keyword
aflp marker
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: CPP-26-89
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||