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GrainGenes Reference Report: PPS-156-20

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Reference	PPS-156-20
Title	Comprehensive sequence analysis of 24,783 barley full-length cDNAs derived from 12 clone libraries.
Journal	Plant Physiology
Year	2011
Volume	156
Pages	20-28
Author	Matsumoto T [ Show all 14 ]
Abstract	Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.
External Databases	Pubmed: 21415278
Locus	AK353559.1 [ Show all 23614 ]
Probe	NIASHv1001A10 [ Show all 23613 ]

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