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GrainGenes Reference Report: TAG-130-495

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Reference	TAG-130-495
Title	Fine mapping of the chromosome 5B region carrying closely linked rust resistance genes Yr47 and Lr52 in wheat.
Journal	Theoretical and Applied Genetics
Year	2017
Volume	130
Pages	495-504
Author	Qureshi N [ Show all 9 ]
Abstract	Fine mapping of Yr47 and Lr52 in chromosome arm 5BS of wheat identified close linkage of the marker sun180 to both genes and its robustness for marker-assisted selection was demonstrated. The widely effective and genetically linked rust resistance genes Yr47 and Lr52 have previously been mapped in the short arm of chromosome 5B in two F3 populations (Aus28183/Aus27229 and Aus28187/Aus27229). The Aus28183/Aus27229 F3 population was advanced to generate an F6 recombinant inbred line (RIL) population to identify markers closely linked with Yr47 and Lr52. Diverse genomic resources including flow-sorted chromosome survey sequence contigs representing the orthologous region in Brachypodium distachyon, the physical map of chromosome arm 5BS, expressed sequence tags (ESTs) located in the 5BS6-0.81-1.00 deletion bin and resistance gene analog contigs of chromosome arm 5BS were used to develop markers to saturate the target region. Selective genotyping was also performed using the iSelect 90K Infinium wheat SNP assay. A set of SSR, STS, gene-based and SNP markers were developed and genotyped on the Aus28183/Aus27229 RIL population. Yr47 and Lr52 are genetically distinct genes that mapped 0.4cM apart in the RIL population. The SSR marker sun180 co-segregated with Lr52 and mapped 0.4cM distal to Yr47. In a high resolution mapping population of 600 F2 genotypes Yr47 and Lr52 mapped 0.2cM apart and marker sun180 was placed 0.4cM distal to Lr52. The amplification of a different sun180 amplicon (195bp) than that linked with Yr47 and Lr52 (200bp) in 204 diverse wheat genotypes demonstrated its robustness for marker-assisted selection of these genes.
External Databases	PubMed: 27866228

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