GrainGenes Reference Report: TAG-122-211
Reference
TAG-122-211
Title
Identification and development of a functional marker of TaGW2 associated with grain weight in bread wheat (Triticum aestivum L.)
Journal
Theoretical and Applied Genetics
Year
2011
Volume
122
Pages
211-223
Author
Su Z Hao C Wang L Dong Y Zhang X
Abstract
The OsGW2 gene is involved in rice grain development, influencing grain width and weight. Its ortholog in wheat, TaGW2, was considered as a candidate gene related to grain development. We found that TaGW2 is constitutively expressed, with three orthologs expressing simultaneously. The coding sequence (CDS) of TaGW2 is 1,275 bp encoding a protein with 424 amino acids, and has a functional domain shared with OsGW2. No divergence was detected within the CDS sequences in the same locus in ten varieties. Genome-specific primers were designed based on the sequence divergence of the promoter regions in the three orthologous genes, and TaGW2 was located in homologous group 6 chromosomes through CS nulli-tetrasomic (NT). Two SNPs were detected in the promoter region of TaGW2-6A, forming two haplotypes: Hap-6A-A (-593A and -739G) and Hap-6A-G (-593G and -769A). A cleaved amplified polymorphic sequence (CAPS) marker was developed based on the -593 A-G polymorphism to distinguish the two haplotypes in TaGW2-6A. This gene was fine mapped 0.6 cM from marker cfd80.2 near the centromere in a recombinant inbred line (RIL) population. Two hundred sixty-five Chinese wheat varieties were genotyped and association analysis revealed that Hap-6A-A was significantly associated with wider grains and higher one-thousand grain weight (TGW) in two crop seasons. qRT-PCR revealed a negative relationship between TaGW2 expression level and grain width. The Hap-6A-A frequencies in Chinese varieties released at different periods showed that it had been strongly positively selected in breeding. In landraces, Hap-6A-A is mainly distributed in southern Chinese wheat regions. Association analysis also indicated that Hap-6A-A not only increased TGW by more than 3 g, but also had earlier heading and maturity. In contrast to Chinese varieties, Hap-6A-G was the predominant haplotype in European varieties; Hap-6A-A was mainly present in varieties released in the former Yugoslavia, Italy, Bulgaria, Hungary and Portugal.
External Databases
http://link.springer.com/article/10.1007%2Fs00122-010-1437-z PubMed: 20838758 https://doi.org/10.1007/s00122-010-1437-z
Gene
TaGW2-6A (Triticum) TaGW2-A1
Map Data
Wheat, Xiaoyan 54 x Jing 411_6A
Probe
Hap-6A-P1 Hap-6A-P2
2 Point Data
TaGW2-6A/Xcfd80
Summarized in
MAS Wheat. Bringing Genomics to the Wheat Fields. Yield. Thousand Grain Weight - Grain Size. TaGW2-A1 MAS Wheat. Marker Assisted Selection in Wheat.
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-122-211
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||