GrainGenes Reference Report: BMB-30-755
Reference
BMB-30-755
Title
Multiple nuclear protein binding to 135 bp subrepeat element of wheat ribosomal DNA intergenic spacer
Journal
Biochemistry and Molecular Biology International
Year
1993
Volume
30
Pages
755-761
Author
Ashapkin V Antoniv T Vanyushin B
Abstract
Specific binding of wheat nuclear proteins to 135 bp subrepeat element of the ribosomal DNA intergenic spacer was studied by electrophoretic mobility shift assays A number of specific DNA-protein complexes are readily formed upon incubation of the labelled 135 bp subrepeat element with wheat nuclear protein extracts Some of these complexes have various DNA-binding specificities as evidenced by their optimal detection in the presence of different non-specific competitor polynucleotides One of the wheat nuclear proteins was found to bind specifically both to 135 bp subrepeat element and an intergenic spacer region located downstream of the transcription initiation site (between +192 and +366 bp) No apparent effects of the 135 bp element methylation with methylase HpaII on protein binding were found
Keyword
binding nucleoproteins ribosomal dna seed triticum aestivum
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: BMB-30-755
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||