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GrainGenes Reference Report: BLT-24-721

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Reference	BLT-24-721
Title	Generation of transgenic wheat plants producing high levels of the osmoprotectant proline
Journal	Biotechnology Letters
Year	2002
Volume	24
Pages	721-725
Author	Sawahel WA Hassan AH
Abstract	Summary: Plasmid DNA (pBI-P5CS), containing the selectable neomycin phosphotransferase-II 'npt II' gene for kanamycin resistance and the reporter beta-glucuronidase 'gus' gene as well as the Vigna aconitifolia delta(1)-pyrroline-5-carboxylate synthetase 'P5CS' cDNA that encodes enzymes required for the biosynthesis of proline, was delivered into wheat plants using Agrobacterium-mediated gene transfer via indirect pollen system. Southern, northern and western blot analysis demonstrated that the foreign gene had been transferred, expressed and integrated into wheat chromosomal DNA. Salinity test indicated that proline acts as an osmoprotectant and its overproduction in transgenic wheat plants results in the increased tolerance to salt
Keyword	agrobacterium-mediated transformation [ Show all 39 ]

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