GrainGenes Reference Report: VIR-208-303
Reference
VIR-208-303
Title
Bent DNA in the large intergenic region of wheat dwarf geminivirus
Journal
Virology
Year
1995
Volume
208
Pages
303-311
Author
Suarez-Lopez P Martinez-Salas E Hernandez P Gutierrez C
Abstract
Wheat dwarf virus (WDV) is a member of the geminivirus group, unique plant DNA viruses which replicate exclusively via dsDNA replication intermediates The large intergenic region (LIR), a nontranscribed regulatory region, contains an inverted repeat with the potential to form a stem-loop structure in which the initiation site for WDV (+)strand (virion-sense) DNA replication has been mapped In this work, we have studied by two-dimensional electrophoresis and by electron microscopy the DNA structure of the WDV LIR and we have identified a DNA sequence which confers a static DNA curvature This is the first report of the occurrence of DNA bending in the regulatory region of a geminivirus genome The bending locus, defined by an 80-bp cluster of A(T) tracts, is located downstream from the potential stem-loop The A(T) tract proximal to the stem-loop structure is separated by 14 bp from the inverted repeat, and deletion of distal A(T) tracts abolished the curvature of the new WDV delta LIR The bending center maps approximately 80 bp downstream from the putative initiation site for (+)strand DNA replication and approximately 70 bp upstream from the TATA box for virion-sense transcription. The possible implications of the DNA bending locus as a regulatory element of WDV DNA replication and/or virion-sense transcription are discussed.
Keyword
dna
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GrainGenes Reference Report: VIR-208-303
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||