Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TPJ-19-173

[Submit comment/correction]

Reference	TPJ-19-173
Title	Transcription activation mediated by the bZIP factor SPA on the endosperm box is modulated by ESBF-1 in vitro.
Journal	Plant Journal
Year	1999
Volume	19
Pages	173-181
Author	Conlan RS Hammond-Kosack M Bevan M
Abstract	Summary: A modified in vitro transcription system has been used to study the function of the cloned bZIP transcription factor SPA and the binding activity ESBF I in activating transcription from the bifactorial endosperm box region of the wheat prolamin LMWG-1D1 gene. Recombinant SPA expressed in Escherichia coli activated transcription from the endosperm box motif, and this was dependent upon the binding of the nuclear protein ESBF I. ESBF I did not activate transcription independently, but potentiated SPA-mediated transcriptional activation. ESBF I is likely to be the equivalent of, or contain the recently characterised DOF class of, Zn-finger protein called WPBF. These data provide new information about the interplay of members of the bZIP and DOF transcription factor families in regulating expression from bifactorial sites found in a variety of plant promoters
External Databases	Pubmed: 10476064
Keyword	dna binding motifs [ Show all 10 ]

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.