GrainGenes Reference Report: TAG-109-800
Reference
TAG-109-800
Title
Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs)
Journal
Theoretical and Applied Genetics
Volume
109
Pages
800-805
Author
Nicot N
[ Show all 8 ]
Abstract
The increasing availability of expressed sequence tags (ESTs) in wheat (Triticum aestivum) and related cereals provides a valuable resource of non-anonymous DNA molecular markers. We examined 170,746 wheat ESTs from the public (International Triticeae EST Cooperative) and Genoplante databases, previously clustered in contigs, for the presence of di- to hexanucleotide simple sequence repeats (SSRs). Analysis of 46,510 contigs identified 3,530 SSRs, which represented 7.5% of the total number of contigs. Only 74% of the sequences allowed primer pairs to be designed, 70% led to an amplification product, mainly of a high quality (68%), and 53% exhibited polymorphism for at least one cultivar among the eight tested. Even though dinucleotide SSRs were less represented than trinucleotide SSRs (15.5% versus 66.5%, respectively), the former showed a much higher polymorphism level (83% versus 46%). The effect of the number and type of repeats is also discussed. The development of new EST-SSRs markers will have important implications for the genetic analysis and exploitation of the genetic resources of wheat and related species and will provide a more direct estimate of functional diversity
Locus
gpw7055 gpw7060 gpw7072 gpw7079 gpw7082
Probe
GPW7072 GPW7082 GPW7079 GPW7060 GPW7055
Keyword
dna primers
[ Show all 9 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-109-800
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||